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人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-His タグ

データシートレビュー関連製品プロトコル
Human CA13 cDNA クローン製品情報
Gene_bank_ref_id:NM_198584.2
cDNA サイズ:789bp
cDNA の説明:Full length Clone DNA of Homo sapiens carbonic anhydrase XIII with C terminal His tag.
遺伝子の別名:CAXIII, FLJ37995, MGC59868, CA13
:Human
ベクター:pCMV3-C-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-His タグ on other vectors
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人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10461-ANRJPY54410
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人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-His タグHG10461-CHJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-Myc タグHG10461-CMJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-HA タグHG10461-CYJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone in cloning vectorHG10461-MJPY18140
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, C-Flag タグHG10461-M-FJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, N-Flag タグHG10461-NFJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, N-His タグHG10461-NHJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, N-Myc タグHG10461-NMJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmid, N-HA タグHG10461-NYJPY47150
人々 Carbonic Anhydrase XIII/CA13 Gene ORF cDNA clone expression plasmidHG10461-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. The CAXIII is a member of the CA family, which owns a globular molecule with high structural similarity to cytosolic isozymes, CAI, II, and III. Recombinant mouse CAXIII showed catalytic activity similar to those of mitochondrial CAV and cytosolic CAI. In human tissues, CAXIII expression was identified in the thymus, small intestine, spleen, prostate, ovary, colon, and testis. In mouse, positive tissues included the spleen, lung, kidney, heart, brain, skeletal muscle, and testis. In conclusion, the predicted amino acid sequence, structural model, distribution, and activity data suggest that CAXIII represents a novel enzyme, which may play important physiological roles in several organs.

参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
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    カタログ: HG10461-CH
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