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人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA タグ

データシートレビュー関連製品プロトコル
Human CA2 cDNA クローン製品情報
Gene_bank_ref_id:NM_000067.2
cDNA サイズ:783bp
cDNA の説明:Full length Clone DNA of Homo sapiens carbonic anhydrase II with C terminal HA tag.
遺伝子の別名:CAII, Car2, CA-II, CA2
:Human
ベクター:pCMV3-C-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA タグ on other vectors
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人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグHG10478-CMJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグHG10478-CMJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA タグHG10478-CYJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone in cloning vectorHG10478-MJPY18135
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag タグHG10478-NFJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-His タグHG10478-NHJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Myc タグHG10478-NMJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-HA タグHG10478-NYJPY47151
人々 Carbonic Anhydrase II Gene ORF cDNA clone expression plasmidHG10478-UTJPY47151
 発現ベクターの詳細情報
Product nameProduct name
背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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