クイック注文

人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-Flag タグ

    データシートレビュー関連製品プロトコル
    人々 ACTL6A cDNA クローン製品情報
    Gene_bank_ref_id:NM_004301.3
    cDNA サイズ:1290bp
    cDNA の説明:Full length Clone DNA of Homo sapiens actin-like 6A with C terminal Flag tag.
    遺伝子の別名:Arp4, ACTL6, BAF53A, INO80K, MGC5382
    :Human
    ベクター:pCMV3-C-FLAG
    Plasmid:
    制限サイト:
    タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
    シーケンスの説明:
    Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
    ( We provide with ACTL6A qPCR primers for gene expression analysis, HP100838 )
    Promoter:Enhanced CMV mammalian cell promoter
    Application:Stable or Transient mammalian expression
    Antibiotic in E.coli:Kanamycin
    Antibiotic in mammalian cell:Hygromycin
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    FLAG Tag Info

    FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

    A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

    The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-Flag タグ on other vectors
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10963-ACGJPY54410
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10963-ACRJPY54410
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10963-ANGJPY54410
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10963-ANRJPY54410
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-Flag タグHG10963-CFJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-His タグHG10963-CHJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-Myc タグHG10963-CMJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-HA タグHG10963-CYJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone in cloning vectorHG10963-MJPY18140
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, C-Flag タグHG10963-M-FJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmidHG10963-M-NJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-Flag タグHG10963-NFJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-His タグHG10963-NHJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-Myc タグHG10963-NMJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmid, N-HA タグHG10963-NYJPY47150
    人々 Actin-like 蛋白質 6A/BAF53a Gene ORF cDNA clone expression plasmidHG10963-UTJPY47150
     発現ベクターの詳細情報
    Product nameProduct name
    Size / Price
    カタログ: HG10963-CF
    定価: 
    価格:      (You Save: )

    Datasheet & Documentation

    Contact Us
    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"