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HIV-1 (group M, subtype B, strain HXB2) nef ORF mammalian expression plasmid, N-His タグ

データシートレビュー関連製品プロトコル
HIV HIV-nef cDNA クローン製品情報
Gene_bank_ref_id:K03455
cDNA サイズ:372bp
cDNA の説明:Full length Clone DNA of HIV-1 (group M, subtype B, strain HXB2) nef with N terminal His tag.
遺伝子の別名:HIV-nef
:HIV
ベクター:pCMV3-N-His
Plasmid:
制限サイト:
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

HIV-1 (group M, subtype B, strain HXB2) nef ORF mammalian expression plasmid, N-His タグ on other vectors
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