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人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-Myc タグ

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Human ITGB1 cDNA クローン製品情報
Gene_bank_ref_id:NM_002211.3
cDNA サイズ:2397bp
cDNA の説明:Full length Clone DNA of Homo sapiens integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), transcript variant 1A with C terminal Myc tag.
遺伝子の別名:CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA
:Human
ベクター:pCMV3-C-Myc
Plasmid:pCMV3-ITGB1-Myc
制限サイト:KpnI + XbaI (6kb + 2.44kb)
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 459T/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Human ITGB1 Gene Plasmid Map
HHuman Integrin beta1 transcript variant 1A ORF mammalian expression plasmid, C-Myc tag
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-GFPSpark タグHG10587-ACGJPY59240
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-OFPSpark タグHG10587-ACRJPY59240
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-GFPSpark タグHG10587-ANGJPY59240
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-OFPSpark タグHG10587-ANRJPY59240
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-Flag タグHG10587-CFJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-His タグHG10587-CHJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-Myc タグHG10587-CMJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, C-HA タグHG10587-CYJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone in cloning vectorHG10587-MJPY18140
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-Flag タグHG10587-NFJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-His タグHG10587-NHJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-Myc タグHG10587-NMJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmid, N-HA タグHG10587-NYJPY51990
人々 ITGB1 / Integrin beta-1 / CD29 transcript variant 1A Gene ORF cDNA clone expression plasmidHG10587-UTJPY51990
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  • HHuman Integrin beta1 transcript variant 1A ORF mammalian expression plasmid, C-Myc tag
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