Her2 / ERBB2 Protein, Human, Recombinant (His Tag)

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Her2 / ERBB2 Protein, Human, Recombinant (His Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA . Immobilized human Erbb2 at 2.5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1.28-32 ng/ml.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Thr 652) of human ErbB2 (NP_004439.2) was fused with the polyhistidine tag at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Thr 23
分子量
The recombinant human ErbB2 comprises 641 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, rh ErbB2 migrates as an approximately 100-110 kDa protein in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Her2 / ERBB2 Protein, Human, Recombinant (His Tag): 画像

Her2 / ERBB2 Protein, Human, Recombinant (His Tag): 別名

CD340 Protein, Human; ENV Protein, Human; ENVW Protein, Human; ERVWE1 Protein, Human; HER-2 Protein, Human; HER-2/neu Protein, Human; HER2 Protein, Human; HERV-7q Protein, Human; HERV-W-ENV Protein, Human; HERV7Q Protein, Human; HERVW Protein, Human; HERVWENV Protein, Human; MLN 19 Protein, Human; MLN19 Protein, Human; NEU Protein, Human; NGL Protein, Human; TKR1 Protein, Human

Her2 / ERBB2 背景情報

Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD34, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.
完全な名称
erb-b2 receptor tyrosine kinase 2
参考文献
  • Krawczyk N, et al. (2009) HER2 status on persistent disseminated tumor cells after adjuvant therapy may differ from initial HER2 status on primary tumor. Anticancer Res. 29(10): 4019-24.
  • Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. coli cytoplasm
    Author
    Markiv, A;Beatson, R;Burchell, J;Durvasula, RV;Kang, AS;
    Year
    2011
    Journal
    BMC Biotechnol.
    Application
    SPR
  • Modified trastuzumab and cetuximab mediate efficient toxin delivery while retaining antibody-dependent cell-mediated cytotoxicity in target cells
    Author
    Gilabert-Oriol, R;Thakur, M;von Mallinckrodt, B;Hug, T;Wiesner, B;Eichhorst, J;Melzig, MF;Fuchs, H;Weng, A;
    Year
    2013
    Journal
    Mol. Pharm.
    Application
    SPR
  • Development of an immunoassay for human epidermal growth factor receptor 2 (HER2)
    Author
    Henanger, KJ;
    Year
    2014
    Journal
    Thesis
  • Engineering of Bispecific Affinity Proteins with High Affinity for ERBB2 and Adaptable Binding to Albumin
    Author
    Nilvebrant, J;Astrand, M;Georgieva-Kotseva, M;Björnmalm, M;Löfblom, J;Hober, S;
    Year
    2014
    Journal
    PLoS ONE
    Application
    remaining tracks
  • Exploring the effects of linker composition on site-specifically modified antibody-drug conjugates
    Author
    AE Albers, AW Garofalo, PM Drake, R Kudirka…
    Year
    2014
    Journal
    European Journal of Medicinal Chemistry
    Application
    ELISA
  • Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates
    Author
    Eletxigerra, U;Martinez-Perdiguero, J;Barderas, R;Pingarrón, JM;Campuzano, S;Merino, S;
    Year
    2016
    Journal
    Anal. Chim. Acta
  • Molecular and hydrodynamic properties of human epidermal growth factor receptor HER2 extracellular domain and its homodimer: Experiments and multi-scale simulations
    Author
    Vega, JF;Ramos, J;Cruz, V;Vicente-Alique, E;Sánchez-Sánchez, E;Sánchez-Fernández, A;Wang, Y;Hu, P;Cortés, J;Martínez-Salazar, J;
    Year
    2017
    Journal
    Biochim. Biophys. Acta
  • Ultra-low fouling methylimidazolium modified surfaces for the detection of HER2 in breast cancer cell lysates
    Author
    Aubé, A;Campbell, S;Schmitzer, AR;Claing, A;Masson, JF;
    Year
    2017
    Journal
    Analyst
  • Single-step Enzymatic Glycoengineering for the Construction of Antibody-cell Conjugates
    Author
    Li, J;Chen, M;Liu, Z;Zhang, L;Felding, B;Lauvau, G;Abadier, M;Ley, K;Wu, P;
    Year
    2018
    Journal
    bioRxiv
    Application
    binding
  • Site-Specific Photoconjugation of Beta-Lactamase Fragments to Monoclonal Antibodies Enables Sensitive Analyte Detection via Split-Enzyme Complementation
    Author
    Yu, F;Alesand, V;Nygren, PÅ;
    Year
    2018
    Journal
    Biotechnol J
    Application
    binding
  • Structural and functional characterization of MBS301, an afucosylated bispecific anti-HER2 antibody
    Author
    Huang, S;Li, F;Liu, H;Ye, P;Fan, X;Yuan, X;Wu, Z;Chen, J;Jin, C;Shen, B;Feng, J;Zhang, B;
    Year
    2018
    Journal
    MAbs
    Application
    SPR
  • High-resolution glycosylation site-engineering method identifies MICA epitope critical for shedding inhibition activity of anti-MICA antibodies
    Author
    Lombana, TN;Matsumoto, ML;Bevers Iii, J;Berkley, AM;Toy, E;Cook, R;Gan, Y;Du, C;Liu, P;Schnier, P;Sandoval, W;Ye, Z;Schartner, JM;Kim, J;Spiess, C;
    Year
    2018
    Journal
    MAbs
    Application
    control
  • Hydrodynamic and electrophoretic properties of Trastuzumab/HER2 complexes. Experiments and modeling.
    Author
    Ramos, J;Vega, J;Cruz, V;Sanchez-Sanchez, E;Cortes, J;Martinez-Salazar, J;
    Year
    2018
    Journal
    bioRxiv
    Application
    binding
  • Constructive approach for synthesis of a functional IgG using a reconstituted cell-free protein synthesis system
    Author
    Murakami, S;Matsumoto, R;Kanamori, T;
    Year
    2019
    Journal
    Sci Rep
    Application
    ELISA and Octet
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