Influenza A H5N1 (A/Duck/Hong Kong/p46/97) Hemagglutinin / HA Protein (His Tag)

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Influenza A H5N1 (A/Duck/Hong Kong/p46/97) Hemagglutinin / HA Protein (His Tag): 製品情報

純度
> 92 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met1-Gln539) of the influenza hemagglutinin (A/Duck/Hong Kong/p46/97 (H5N1)) (AAF02306.1) (HA1+HA2, uncleaved), cleavage site mutated (RRRKKR-TETR) was fused with a polyhistidine tag at the C-terminus.
GenBank No.
発現ホスト
HEK293 Cells
H5N1
予測N末端
Arg 23
分子量
The secreted recombinant influenza A H5N1 HA (A/Duck/Hong Kong/p46/97 (H5N1)) comprises 526 amino acids and has a predicted molecular mass of 60 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, 750mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Influenza A H5N1 (A/Duck/Hong Kong/p46/97) Hemagglutinin / HA Protein (His Tag): 画像

Hemagglutinin / HA 背景情報

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
完全な名称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37

H5N1 Hemagglutinin / HA タンパク質

H3N2 Hemagglutinin / HA タンパク質

H1N1 Hemagglutinin / HA タンパク質

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