Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag)

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Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Gln 528) of the influenza A hemagglutinin (A/Puerto Rico/8/34 (H1N1)) (ABD77675.1), was expressed, fused with a C-terminal polyhistidine tag.
GenBank No.
発現ホスト
HEK293 Cells
H1N1
予測N末端
Asp 18
分子量
The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Influenza A H1N1 (A/Puerto Rico/8/34) Hemagglutinin / HA Protein (His Tag): 画像

Hemagglutinin / HA 背景情報

The influenza viral Hemagglutinin (HA) protein is a homo trimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
完全な名称
Harvey rat sarcoma viral oncogene homolog
参考文献
  • White JM, Hoffman LR, Arevalo JH, et al. (1997). ""Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin"". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.
  • Suzuki Y (March 2005). ""Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses"". Biol. Pharm. Bull. 28 (3): 399–408.
  • Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). ""Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential"". Avian Dis. 40 (2): 425–37

H5N1 Hemagglutinin / HA タンパク質

H3N2 Hemagglutinin / HA タンパク質

H1N1 Hemagglutinin / HA タンパク質

  • A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus
    Author
    Wu, CY;Yeh, YC;Chan, JT;Yang, YC;Yang, JR;Liu, MT;Wu, HS;Hsiao, PW;
    Year
    2012
    Journal
    PLoS ONE
    Application
    WB analysis of VLP-induced humoral immunity against HA (detection antigen)immune sera
  • Molecular adjuvant Ag85A enhances protection against influenza A virus in mice following DNA vaccination
    Author
    Dai, J;Pei, D;Wang, B;Kuang, Y;Ren, L;Cao, K;Wang, H;Zuo, B;Shao, J;Li, S;Li, H;Li, M;
    Year
    2012
    Journal
    Viruses
  • A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus
    Author
    Dai, J;Pei, D;Wang, B;Kuang, Y;Ren, L;Cao, K;Zuo, B;Shao, J;Li, S;Jiang, Z;Li, H;Li, M;
    Year
    2013
    Journal
    Virol. J.
    Application
    WB to detect HA expression
  • Whole-Virus Screening to Develop Synbodies for the Influenza Virus
    Author
    Gupta, N;Lainson, J;Domenyuk, V;Zhao, ZG;Johnston, SA;Diehnelt, CW;
    Year
    2016
    Journal
    Bioconjug. Chem.
    Application
    SPR
  • A DNA vaccine that targets hemagglutinin to antigen presenting cells protects mice against H7 influenza
    Author
    Andersen, TK;Zhou, F;Cox, R;Bogen, B;Grødeland, G;
    Year
    2017
    Journal
    J. Virol.
  • Kallikrein-related peptidase 5 contributes to H3N2 influenza virus infection in human lungs
    Author
    Magnen, M;Gueugnon, F;Guillon, A;Baranek, T;Thibault, VC;Petit-Courty, A;de Veer, SJ;Harris, J;Humbles, AA;Si-Tahar, M;Courty, Y;
    Year
    2017
    Journal
    J. Virol.
    Application
    substrate
  • Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells
    Author
    Gaya, M;Barral, P;Burbage, M;Aggarwal, S;Montaner, B;Warren Navia, A;Aid, M;Tsui, C;Maldonado, P;Nair, U;Ghneim, K;Fallon, PG;Sekaly, RP;Barouch, DH;Shalek, AK;Bruckbauer, A;Strid, J;Batista, FD;
    Year
    2017
    Journal
    Cell
    Application
    binding (ELISA)
  • Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses
    Author
    Pardi, N;Hogan, MJ;Naradikian, MS;Parkhouse, K;Cain, DW;Jones, L;Moody, MA;Verkerke, HP;Myles, A;Willis, E;LaBranche, CC;Montefiori, DC;Lobby, JL;Saunders, KO;Liao, HX;Korber, BT;et al.
    Year
    2018
    Journal
    J. Exp. Med.
    Application
    immunization
  • Simultaneous Targeting of Multiple Hemagglutinins to APCs for Induction of Broad Immunity against Influenza
    Author
    Anderson, AM;Baranowska-Hustad, M;Braathen, R;Grodeland, G;Bogen, B;
    Year
    2018
    Journal
    J. Immunol.
    Application
    ELISA
  • The Cranberry Extract Oximacro® Exerts in vitro Virucidal Activity Against Influenza Virus by Interfering With Hemagglutinin
    Author
    Luganini, A;Terlizzi, ME;Catucci, G;Gilardi, G;Maffei, ME;Gribaudo, G;
    Year
    2018
    Journal
    Front Microbiol
    Application
    binding
  • Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules
    Author
    Andersen, TK;Huszthy, PC;Gopalakrishnan, RP;Jacobsen, JT;Fauskanger, M;Tveita, AA;Grødeland, G;Bogen, B;
    Year
    2019
    Journal
    NPJ Vaccines
    Application
    ELISA
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