Anti-GST tag Antibody

Anti-GST tag Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-GST tag Antibody
検証済のアプリケーション
WB,IP
交差反応
Reacts with: Schistosoma japonicum
特異性
Schistosoma japonicum GST tag
No cross-reactivity with E.coli cell lysate in ELISA.
免疫原
Recombinant GST protein
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Glutathione S-transferase (GST). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #6C10G4
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GST tag Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1/1000-1/10000
IP 1-4 μl/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GST tag Antibody (Mouse Monoclonal 抗体) の画像

Anti-GST Tag was immunoprecipitated using:

Lane A: GST-ARG1-Flag transfected 293 cell lysate (0.5mg)

Lane B: Flag-ARG1-GST transfected 293 cell lysate (0.5mg)

 

2 μg anti-HA Tag mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilution

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human ERK2.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (100ng)

Lane B: GST-ERK2 (Recombinant protein, Cat#10030-H09B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion protein human USP7.

Anti-GST Tag mouse monoclonal antibody at 1:4000

Lane A: GST-USP7 (Recombinant protein, Cat#11681-H20B) (100ng)

Lane B: GST-USP7 (Recombinant protein, Cat#11681-H20B) (50ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by 293 cell.

Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500

Lane A: GST-FABP4-flag transfected 293 Cell Lysate (30ug)

Lane B: flag-FABP4-GST transfected 293 Cell Lysate (30ug)

Lane C: flag-AGR1-GST transfected 293 Cell Lysate (30ug)

Lane D: Negative control

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Recognize GST tag fusion proteins which expressed by E. coli.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250

Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)

Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)

Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)

Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)

Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)

Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)

Lane K: Empty vecter (0.5ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000

Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)

Lane C: Empty vecter (0.1ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000

Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)

Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)

Lane C: Empty vecter (0.2ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

カートに追加しました カートに追加しませんでした