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GFPSpark® & OFPSpark® tagged Expression ORF Clones

Sinobiological has constructed expression-ready plasmids which the target gene is tagged with GFPSpark®/ OFPSpark® in N- or C-terminal. Fluorescent proteins as genetically encoded tools are used extensively by life scientists. Almost all endogenous proteins of interest can be fused to fluorescent proteins. The fusion proteins can direct visualization of the organelles or structures by fluorescence microscopy in mammalian cells.
The main applications of fluorescent proteins include assay promoter activity,organelle marker,protein interaction,drug screening,drganism labeling,sensors.


Sino Biological Inc. recommend with organelle marker expression vector:

1) GFP or OFP fluorescent colors for choice
2) Easy labeling of cell compartments and proteins
3) Perfect for study of localization and trafficking
4) No antibody or chemical treatment required

Fluorescent Protein

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and gene was first cloned in 1992. The first demonstration of its utility as a fluorescent tag for in vivo labeling in 1994,and since then a keen interest in the structure, biochemistry, and biophysics of GFP-like fluorescent proteins, which resulted in an avalanche of scientific publications on FPs and their applications to solve basic problems in molecular and cell biology.Sinobio has self-developed Green fluorescent protein GFPSpark® and Red (orange) fluorescent protein OFPSpark®.

 
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cDNA / clones

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Lentiviral Vector

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and gene was first cloned in 1992. The first demonstration of its utility as a fluorescent tag for in vivo labeling in 1994,and since then a keen interest in the structure, biochemistry, and biophysics of GFP-like fluorescent proteins, which resulted in an avalanche of scientific publications on FPs and their applications to solve basic problems in molecular and cell biology.Sinobio has self-developed Green fluorescent protein GFPSpark® and Red (orange) fluorescent protein OFPSpark®.

OFPSpark® is a red (orange) fluorescent protein (excitation/emission maxima are 549 and 566nm, respectively) derived from DsRed. Possessing high photostability and pH stability, OFPSpark® is more than twice brighter than mOrange2. Fast OFPSpark® maturation makes it clearly detectable in mammalian cells as early as within 8hrs after transfection.
- Bright fluorescence
- High pH-stability
- Fast maturation
- Recommended for gene expression analysis, cell labeling and organelle labeling

Green Fluorescent Protein GFPSpark® Orange/Red Fluorescencent Protein OFPSpark®

GFPSpark® & OFPSpark® tag cDNA ORF

  • ● Higher protein expression level: enhanced CMV promoter
    ● multiple tags for choice: C-,N-GFPSpark®/OFPSpark®
    ● full-length sequence confirmed
  • ● mammalian expression, stable or transient
    ● expression validated
Here are the Expression cDNA ORF Maps:  

GFPSpark® & OFPSpark® tag cDNA ORF expression validated

GFPSpark® comparison with EGFP: Brighter fluorescence

OFPSpark® comparison with commercial orange RFP: Brighter fluorescence

A) pCMV3-GFPSpark® and pCMV3-EGFP recombinant plasmids transiently expression in 293 H cell.The green fluorescent signals can be detected in cytoplasm under the excitation channel of 455~495 nm by Microscopy. GFPSpark® gives the brighter signal 24h after transfection.
B) The TUBB gene tagged with GFPSpark® and EGFP on pCMV3 vector
C) The TOMM20 gene tagged with GFPSpark® and EGFP on pCMV3 vector
These different expression vector transfected Hela cells transiently. After 48h, the strong green fluorescent signals can be detected by laser scanning confocal microscope(Nikon).The fluorecene intensity of GFPSpark® is better than EGFP.
OFPSpark®,commercial orange Red recombinant plasmids were constructed on pCMV3 vector.Transiently transfected 293H cells, the red and orange fluorescent signals can be detected in cytoplasm under the excitation channel of 532.5~587.5 nm, OFPSpark® gives the brighter signal 48h after transfection.

GFP/OFP-tagged Expression-ready cDNA ORF Clones for Organelle Marker

The recombinant plasmids containing different subcellular localization genes were constructed on pCMV3 vector.These different expression vector transfected Hela cells transiently. After 48h, the fluorescent signals can be detected by laser scanning confocal microscope(Nikon) (GFPSpark® excitation/ emission max = 487 / 508 nm, OFPSpark® excitation/emission maxima =549 / 566 nm).

HG11111-ANG HG11215-ACG HG12063-ACG HG11277-ACG
HG12282-ACR HG14013-ANG HG11626-ACR HG11263-ACR

More about Organelle Marker GFP/OFP-tagged ecpression ready cDNA PRF

GFPSpark® & OFPSpark® tag cDNA ORF list

             
Autophagosome
ATG12/Apg12
ATG12/Apg12
LC3A/MAP1LC3A
LC3A/MAP1LC3A
LC3B/MAP1LC3B
LC3B/MAP1LC3B
ARHI/DIRAS3
ARHI/DIRAS3
GFPSpark® fusion with ATG12 N terminal
OFPSpark® fusion with ATG12 N terminal
GFPSpark® fusion with MAP1LC3A N terminal
OFPSpark® fusion with MAP1LC3A C terminal
GFPSpark® fusion with MAP1LC3B C terminal
OFPSpark® fusion with MAP1LC3B N terminal
GFPSpark® fusion with DIRAS3 N terminal
OFPSpark® fusion with DIRAS3 N terminal
HG11111-ANG
HG11111-ANR
HG14322-ANG
HG14322-ACR
HG14555-ACG
HG14555-ANR
HG14239-ANG
HG14239-ANR
Green
Orange/Red
Green
Orange/Red
Green
Orange/Red
Green
Orange/Red
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Full list of GFPSpark® & OFPSpark® tag cDNA ORF of Organelle Marker

Fluorescent Protein Related Products

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