After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

クイック注文

Text Size:AAA

フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-HA タグ

データシートレビュー関連製品プロトコル
Ferret IFNG cDNA クローン製品情報
Gene_bank_ref_id:AB300566.1
cDNA サイズ:501bp
cDNA の説明:Full length Clone DNA of Ferret Interferon gamma with N terminal HA tag.
遺伝子の別名:IFNG
:Ferret
ベクター:pCMV3-SP-N-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-HA タグ on other vectors
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-GFPSpark タグFG60007-ACGJPY54405
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-OFPSpark タグFG60007-ACRJPY54405
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-Flag タグFG60007-CFJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-His タグFG60007-CHJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-Myc タグFG60007-CMJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-HA タグFG60007-CYJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone in cloning vectorFG60007-GJPY18135
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, C-His タグFG60007-G-HJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-Flag タグFG60007-NFJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-His タグFG60007-NHJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-Myc タグFG60007-NMJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmid, N-HA タグFG60007-NYJPY47151
フェレット Interferon Gamma/IFN gamma/IFNG Gene ORF cDNA clone expression plasmidFG60007-UTJPY47151
 発現ベクターの詳細情報
Product nameProduct name
背景

IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

参考文献
  • Gray P W, et al. (1982) Structure of the human immune interferon gene. Nature. 298: 859-63.
  • Taya Y, et al. (1982) Cloning and structure of the human immune interferon-gamma chromosomal gene. EMBO J. 1: 953-8.
  • Goshima N, et al. (2008) Human protein factory for converting the transcriptome into an in vitro-expressed proteome. Nomura N Nat Methods. 5: 1011-7.
  • Thiel DJ, et al. (2000) Observation of an unexpected third receptor molecule in the crystal structure of human interferon-gamma receptor complex. Structure. 8 (9): 927-36.
  • Naylor SL, et al. (1983) Human immune interferon gene is located on chromosome 12. J Exp Med. 157 (3): 1020-7.
  • Schoenborn JR, et al. (2007) Regulation of interferon-gamma during innate and adaptive immune responses. Adv Immunol. 96: 41-101.
  • Size / Price
    カタログ: FG60007-NY
    定価: 
    価格:      (You Save: )
    Contact Us
    画像
        All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
        注意:すべての製品は、"研究目的のみに使用するものであり、診断または治療目的に使用するものではありません"