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フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Ferret ENO1 cDNA クローン製品情報
Gene_bank_ref_id:XM_004768617.1
cDNA サイズ:1305bp
cDNA の説明:Full length Clone DNA of Mustela putorius furo (sub-species: furo) enolase 1, (alpha) with N terminal Flag tag.
遺伝子の別名:ENO1
:Ferret
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-GFPSpark タグFG60094-ACGJPY54410
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-OFPSpark タグFG60094-ACRJPY54410
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-GFPSpark タグFG60094-ANGJPY54410
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-OFPSpark タグFG60094-ANRJPY54410
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Flag タグFG60094-CFJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-His タグFG60094-CHJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-Myc タグFG60094-CMJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, C-HA タグFG60094-CYJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone in cloning vectorFG60094-GJPY18140
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Flag タグFG60094-NFJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-His タグFG60094-NHJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-Myc タグFG60094-NMJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmid, N-HA タグFG60094-NYJPY47150
フェレット ENO1 / Enolase 1 / alpha-enolase Gene ORF cDNA clone expression plasmidFG60094-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
参考文献
  • Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74.
  • Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7.
  • Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6.
  • Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.
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    カタログ: FG60094-NF
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