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フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag タグ

データシートレビュー関連製品プロトコル
Ferret CA2 cDNA クローン製品情報
Gene_bank_ref_id:XM_004766964.1
cDNA サイズ:783bp
cDNA の説明:Full length Clone DNA of Mustela putorius furo (sub-species: furo) carbonic anhydrase II with N terminal Flag tag.
遺伝子の別名:CA2
:Ferret
ベクター:pCMV3-N-FLAG
Plasmid:
制限サイト:
タグ シーケンス:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag タグ on other vectors
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-GFPSpark タグFG60084-ACGJPY54410
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-OFPSpark タグFG60084-ACRJPY54410
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-GFPSpark タグFG60084-ANGJPY54410
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-OFPSpark タグFG60084-ANRJPY54410
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Flag タグFG60084-CFJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-His タグFG60084-CHJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグFG60084-CMJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA タグFG60084-CYJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone in cloning vectorFG60084-GJPY18140
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag タグFG60084-NFJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-His タグFG60084-NHJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Myc タグFG60084-NMJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-HA タグFG60084-NYJPY47150
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmidFG60084-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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    カタログ: FG60084-NF
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