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フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグ

データシートレビュー関連製品プロトコル
Ferret CA2 cDNA クローン製品情報
Gene_bank_ref_id:XM_004766964.1
cDNA サイズ:783bp
cDNA の説明:Full length Clone DNA of Mustela putorius furo (sub-species: furo) carbonic anhydrase II with C terminal Myc tag.
遺伝子の別名:CA2
:Ferret
ベクター:pCMV3-C-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグ on other vectors
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-GFPSpark タグFG60084-ACGJPY54405
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-OFPSpark タグFG60084-ACRJPY54405
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-GFPSpark タグFG60084-ANGJPY54405
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-OFPSpark タグFG60084-ANRJPY54405
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Flag タグFG60084-CFJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-His タグFG60084-CHJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-Myc タグFG60084-CMJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, C-HA タグFG60084-CYJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone in cloning vectorFG60084-GJPY18135
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Flag タグFG60084-NFJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-His タグFG60084-NHJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-Myc タグFG60084-NMJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmid, N-HA タグFG60084-NYJPY47151
フェレット Carbonic Anhydrase II Gene ORF cDNA clone expression plasmidFG60084-UTJPY47151
 発現ベクターの詳細情報
Product nameProduct name
背景

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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    カタログ: FG60084-CM
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