EGFR Protein, Human, Recombinant (Fc Tag)

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EGFR Protein, Human, Recombinant (Fc Tag): 製品情報

純度
> 97 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA.
Immobilized recombinant human EGF at 10 μg/ml (100 μl/well) can bind human EGFR with a linear range of 0.64-400 ng/ml.
タンパク質の構築
A DNA sequence encoding the extracellular domain (Met 1-Gly 645) of human EGFR (NP_005219) was fused with the Fc region of human IgG1 at the C-terminus.
NP No.
発現ホスト
HEK293 Cells
Human
予測N末端
Leu 25
分子量
The recombinant human EGFR/Fc chimera is a homodimeric protein. The reduced monomer consists of 860 amino acids and has a calculated molecular mass of 95 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EGFR Protein, Human, Recombinant (Fc Tag): 画像

EGFR Protein, Human, Recombinant (Fc Tag): 別名

ERBB Protein, Human; ERBB1 Protein, Human; HER1 Protein, Human; mENA Protein, Human; NISBD2 Protein, Human; PIG61 Protein, Human

EGFR 背景情報

As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
完全な名称
epidermal growth factor receptor
参考文献
  • Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
  • Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
  • Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
  • Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains
    Author
    Alvarez-Cienfuegos, A;Nuñez-Prado, N;Compte, M;Cuesta, AM;Blanco-Toribio, A;Harwood, SL;Villate, M;Merino, N;Bonet, J;Navarro, R;Muñoz-Briones, C;Sørensen, KM;Mølgaard, K;Oliva, B;Sanz, L;Blanco, FJ;Alvarez-Vallina, L;
    Year
    2016
    Journal
    Sci Rep
    Application
    binding
  • Plasma-Generated OH Radical Production for Analyzing Three-Dimensional Structure in Protein Therapeutics
    Author
    Minkoff, BB;Blatz, JM;Choudhury, FA;Benjamin, D;Shohet, JL;Sussman, MR;
    Year
    2017
    Journal
    Sci Rep
    Application
    test activity
  • A comparative analysis of EGFR-targeting antibodies for gold nanoparticle CT imaging of lung cancer
    Author
    Ashton, JR;Gottlin, EB;Patz, EF;West, JL;Badea, CT;
    Year
    2018
    Journal
    PLoS ONE
    Application
    binding
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