EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag)

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EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag): 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured by its binding ability in a functional ELISA.
Immobilized zebrafish EFNB2A-His at 10 μg/ml (100 μl/well) can bind human EphB4-Fc (Cat:10235-H02H), The EC50 of human EphB4-Fc (Cat:10235-H02H) is 15.5-36.3 ng/ml.
タンパク質の構築
A DNA sequence encoding the zebrafish EFNB2A (O73874) (Met 1-Ala222) was expressed with a polyhistidine tag at the C-terminus.
NCBI Reference No.
発現ホスト
HEK293 Cells
Danio rerio (zebrafish)
予測N末端
Leu 25
分子量
The recombinant zebrafish EFNB2A consists of 209 amino acids and has a calculated molecular mass of 23.3 kDa. It migrates as an 35-40 KDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile PBS, PH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag): 画像

EFNB2A Protein, Danio rerio (zebrafish), Recombinant (His Tag): 別名

efnb2 Protein, Danio rerio (zebrafish); EFNB2A Protein, Danio rerio (zebrafish); id:ibd5072 Protein, Danio rerio (zebrafish)

EFNB2A 背景情報

Efnb2a, also known as ephrin B2a, belongs to the ephrin family. Members of this family are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Efnb2a binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Efnb2a, together with ephb4 may play a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration.
参考文献
  • Shaw KM. et al., 2006, Dev Dyn. 235 (7): 1753-60.
  • Kemp HA. et al., 2009, Dev Biol. 327 (2): 313-26.
  • Patten SA. et al., 2012, PLoS One. 7 (2): e31650.
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