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カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Myc タグ

データシートレビュー関連製品プロトコル
Cynomolgus GOT1 cDNA クローン製品情報
Gene_bank_ref_id:NM_001284930.1
cDNA サイズ:1242bp
cDNA の説明:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) Aspartate aminotransferase, cytoplasmic with N terminal Myc tag.
遺伝子の別名:cCAT, cAspAT
:Cynomolgus
ベクター:pCMV3-N-Myc
Plasmid:
制限サイト:
タグ シーケンス:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Myc タグ on other vectors
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグCG90649-ACGJPY54410
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグCG90649-ACRJPY54410
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグCG90649-ANGJPY54410
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグCG90649-ANRJPY54410
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Flag タグCG90649-CFJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-His タグCG90649-CHJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-Myc タグCG90649-CMJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, C-HA タグCG90649-CYJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone in cloning vectorCG90649-GJPY18140
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Flag タグCG90649-NFJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-His タグCG90649-NHJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-Myc タグCG90649-NMJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmid, N-HA タグCG90649-NYJPY47150
カニクイザル Aspartate aminotransferase / GOT1 Gene ORF cDNA clone expression plasmidCG90649-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

Aspartate aminotransferase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, aspartate aminotransferase and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. There is a rare in-frame deletion in aspartate aminotransferase gene, which inactivates cytosolic aspartate aminotransferase(cAST) enzyme in the Old Order Amish. This may help to understand structure and function of the enzyme and would be useful for predicting serum aspartate AST levels.

参考文献
  • Shen H, et al. (2011) Genome-wide association study identifies genetic variants in GOT1 determining serum aspartate aminotransferase levels. J Hum Genet. 56(11):801-5.
  • Doonan S, et al. (1985) Structural and genetic relationships between cytosolic and mitochondrial isoenzymes. Int J Biochem. 16(12):1193-9.
  • Panteghini M. (1990) Aspartate aminotransferase isoenzymes. Clin Biochem. 23(4):311-9.
  • Bousquet-Lemercier B, et al. (1990) Properties of human liver cytosolic aspartate aminotransferase mRNAs generated by alternative polyadenylation site selection. Biochemistry. 29(22):5293-9.
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