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カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-HA タグ

データシートレビュー関連製品プロトコル
Cynomolgus CHN1 cDNA クローン製品情報
Gene_bank_ref_id:AB173631.1
cDNA サイズ:1005bp
cDNA の説明:Full length Clone DNA of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) chimerin 1 with C terminal HA tag.
遺伝子の別名:CHN1
:Cynomolgus
ベクター:pCMV3-C-HA
Plasmid:
制限サイト:
タグ シーケンス:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
シーケンスの説明:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-HA タグ on other vectors
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグCG90836-ACGJPY54410
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグCG90836-ACRJPY54410
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark タグCG90836-ANGJPY54410
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark タグCG90836-ANRJPY54410
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-Flag タグCG90836-CFJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-His タグCG90836-CHJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-Myc タグCG90836-CMJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, C-HA タグCG90836-CYJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone in cloning vectorCG90836-GJPY18140
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-Flag タグCG90836-NFJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-His タグCG90836-NHJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-Myc タグCG90836-NMJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmid, N-HA タグCG90836-NYJPY47150
カニクイザル CHN1 / chimerin 1 Gene ORF cDNA clone expression plasmidCG90836-UTJPY47150
 発現ベクターの詳細情報
Product nameProduct name
背景

CHN1, also known as chimerin 1, is a TPase-activating protein for ras-related p21-rac and a phorbol ester receptor. It is predominantly expressed in neurons, and plays an important role in neuronal signal-transduction mechanisms. CHN1 is involved in the assembly of neuronal locomotor circuits as a direct effector of EPHA4 in axon guidance. The CHN1 gene provides instructions for making two very similar proteins called α1-chimaerin and α2-chimaerin. These proteins play an important role in the early development of the nervous system. In particular, they help regulate complex chemical signaling pathways during the formation and development of nerve cells (neurons). These proteins help guide the growth of axons and dendrites, which are specialized extensions of neurons that transmit and receive nerve impulses throughout the nervous system.

参考文献
  • Miyake N. et al, 2010, Am J Med Genet A. 152 (1): 215-7.
  • Miyake N. et al., 2011, Invest Ophthalmol Vis Sci. 52 (9): 6321-8.
  • Volk AE. et al., 2010, Graefes Arch Clin Exp Ophthalmol. 248 (9): 1351-7.
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    カタログ: CG90836-CY
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