|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||CG90178-ACG|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||CG90178-ACR|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-Flag タグ||CG90178-CF|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-His タグ||CG90178-CH|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-Myc タグ||CG90178-CM|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, C-HA タグ||CG90178-CY|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone in cloning vector||CG90178-G|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, N-Flag タグ||CG90178-NF|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, N-His タグ||CG90178-NH|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, N-Myc タグ||CG90178-NM|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid, N-HA タグ||CG90178-NY|
|赤毛猿 CD8B / P37 / LEU2 Gene ORF cDNA clone expression plasmid||CG90178-UT|
CD8B (CD8b molecule), also known as P37 and LEU2, contains 1 Ig-like V-type (immunoglobulin-like) domain. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. P37 gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified. CD8 is thought to play a role in the process of T-cell mediated killing.