|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-GFPSpark タグ||CG90173-ACG|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-OFPSpark タグ||CG90173-ACR|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-Flag タグ||CG90173-CF|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-His タグ||CG90173-CH|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-Myc タグ||CG90173-CM|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, C-HA タグ||CG90173-CY|
|赤毛猿 CD1B Gene ORF cDNA clone in cloning vector||CG90173-G|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, N-Flag タグ||CG90173-NF|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, N-His タグ||CG90173-NH|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, N-Myc タグ||CG90173-NM|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid, N-HA タグ||CG90173-NY|
|赤毛猿 CD1B Gene ORF cDNA clone expression plasmid||CG90173-UT|
CD1B contains 1 Ig-like (immunoglobulin-like) domain and belongs to the CD1 family. CD1 family members are transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. During protein synthesis and maturation, they bind endogenous lipids that are replaced by lipid or glycolipid antigens when the proteins are internalized and pass through endosomes, before trafficking back to the cell surface. CD1B localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail, and requires vesicular acidification to bind lipid antigens.. It is expressed on cortical thymocytes, epidermal Langerhans cells, dendritic cells, on certain T-cell leukemias, and in various other tissues. CD1B is an antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.