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Cyclin A1 / CCNA1 抗体, マウスモノクローナル抗体

データシートレビュー関連製品プロトコル
Human CCNA1 抗体製品情報
免疫原:Recombinant Human Cyclin A1 / CCNA1 protein (Catalog#11013-H07B)
Clone ID:06
Ig タイプ:Mouse IgG2b
濃度:
エンドトキシン:
製剤:0.2 μm filtered solution in PBS with 5% trehalose
調製:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Cyclin A1 / CCNA1 (rh Cyclin A1 / CCNA1; Catalog#11013-H07B; NP_003905.1; Met1-Gln465). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human CCNA1 抗体使用ガイド
特異度:Human Cyclin A1 / CCNA1
アプリケーション:ICC/IF, IF, FCM

FCM: 0.5-2 μg/Test

ICC/IF: 10-25 μg/mL

保存:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CCNA1 Antibody IF Application Image
Cyclin A1 / CCNA1 Antibody, Mouse MAb, Immunofluorescence
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Caption:
Immunofluorescence staining of Human CCNA1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CCNA1 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Human CCNA1 Antibody FC Application Image
Cyclin A1 / CCNA1 Antibody, Mouse MAb
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Caption:
Flow cytometric analysis of Human CCNA1 expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human CCNA1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other CCNA1 Antibody Products
Cyclin A1/CCNA1 背景

Cyclin A1 is a member of the highly conserved cyclin family that is characterized by a dramatic periodicity in protein abundance, and belongs to the A-type cyclin subfamily. The mammalian A-type cyclin family consists of two members: cyclin A1 and cyclin A2. Different cyclins exhibit distinct expression. Cyclin A1 is expressed in mice exclusively in the germ cell lineage and high rate of cyclinA1 is found in human testis and certain myeloid leukaemia cells. Cyclin A1 is primarily function in the control of meiosis. It serves as regulator subunits binding to cyclin-dependent kinase 1 (Cdk1) and cyclin-dependent kinase 2 (Cdk2), which give two different kinase activities, one appearing in S phase, the other in G2. Through this, cyclin A1 operate the entry and progression in cell cycle. High frequency of cyclin A1 overexpression has been observed in acute myelocytic leukemias, especially those that are at the promyelocyte and myeloblast stages of development.

Human Cyclin A1/CCNA1 参考文献
  • Yang R, et al. (1999) Functions of Cyclin A1 in the cell cycle and its interactions with transcription factor E2F-1 and the Rb family of proteins. Molecular and Cellular biology. 19 (3): 2400-7.
  • Yang R, et al. (1999) Cyclin A1 expression in leukemia and normal hematopoietic cells. Blood. 93 (6): 2067-74.
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    カタログ: 11013-MM06-50
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    • Cyclin A1 / CCNA1 Antibody, Mouse MAb
    • Cyclin A1 / CCNA1 Antibody, Mouse MAb, Immunofluorescence
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