|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine FGF21 ORF mammalian expression plasmid, C-GFPSpark tag||DG70038-ACG|
|Canine FGF21 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70038-ACR|
|Canine FGF21 ORF mammalian expression plasmid, C-Flag tag||DG70038-CF|
|Canine FGF21 ORF mammalian expression plasmid, C-His tag||DG70038-CH|
|Canine FGF21 ORF mammalian expression plasmid, C-Myc tag||DG70038-CM|
|Canine FGF21 ORF mammalian expression plasmid, C-HA tag||DG70038-CY|
|Canine FGF21 Gene cDNA clone plasmid||DG70038-G|
|Canine FGF21 ORF mammalian expression plasmid, N-Flag tag||DG70038-NF|
|Canine FGF21 ORF mammalian expression plasmid, N-His tag||DG70038-NH|
|Canine FGF21 ORF mammalian expression plasmid, N-Myc tag||DG70038-NM|
|Canine FGF21 ORF mammalian expression plasmid, N-HA tag||DG70038-NY|
|Canine FGF21 natural ORF mammalian expression plasmid||DG70038-UT|
Fibroblast growth factor 21 (FGF21) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 has a hydrophobic amino terminus, which is a typical signal sequence, and appears to be a secreted protein. The metabolic regulator fibroblast growth factor 21 (FGF21) has antidiabetic properties in animal models of diabetes and obesity. FGF21 is a novel adipokine associated with obesity-related metabolic complications in humans. The paradoxical increase of serum FGF21 in obese individuals, which may be explained by a compensatory response or resistance to FGF21, warrants further investigation. FGF-21, which we have identified as a novel metabolic factor, exhibits the therapeutic characteristics necessary for an effective treatment of diabetes.