Anti-Calbindin Antibody

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Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Calbindin Antibody
検証済のアプリケーション
WB,IHC-P,ICC/IF,IF,IP
交差反応
Reacts with: Human
Predicted to React with: Rat (Species predicted to react based on 100% sequence homology)
特異性
Human Calbindin
免疫原
A synthetic peptide corresponding to the N-terminus of the Human Calbindin
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Calbindin, and purified by antigen affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
IHC-P 1:500-1:2000
ICC-IF 1:300-1:10000
IP 4-8 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Calbindin Antibody (Rabbit Polyclonal 抗体) の画像

Immunofluorescence staining of CALB1 in 293 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human CALB1 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunochemical staining of human CALB1 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

CALB1 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg 293T Whole Cell Lysate

4 µL anti-CALB1 rabbit polyclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-CALB1 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 30 kDa

Anti-CALB1 rabbit polyclonal antibody at 1:500 dilution.

Lane A: Mosue kidney tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:30 kDa

Observed band size:27 kDa

Anti-Calbindin Antibody: 別名

Anti-CALB Antibody; Anti-D-28K Antibody
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