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人々 IL8Rb/CXCR2 Gene ORF cDNA clone expression plasmid, C-His タグ

  • Human CXCR2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
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人々 IL8Rb/CXCR2 cDNA クローン製品情報
Gene_bank_ref_id:NM_001557.2
cDNA サイズ:1083bp
cDNA の説明:Full length Clone DNA of interleukin 8 receptor, beta with His tag.
遺伝子の別名:CD182, CXCR2, IL8R2, IL8RA, CMKAR2, CDw128b
:Human
ベクター:pCMV/hygro-His
Plasmid:pCMV-CXCR2-10his
制限サイト:KpnI + XhoI (5.5kb + 1.11kb)
タグ シーケンス:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
シーケンスの説明:Identical with the Gene Bank Ref.ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with CXCR2 qPCR primers for gene expression analysis, HP100702 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
保存:The lyophilized plasmid can be stored at room temperature for three months.
人々 IL8Rb/CXCR2 Gene Plasmid Map
Human CXCR2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged
pCMV/hygro-HIs Vector Information
 
Vector Name pCMV/hygro-His
Vector Size 5687bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive ,Stable / Transient
Promoter CMV
Antibiotic Resistance Ampicillin
Selection In Mammalian Cells Hygromycin
Protein Tag His
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

Schematic of pCMV/hygro-His Multiple Cloning Sites

His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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