B7-1 Protein, Mouse, Recombinant (Fc Tag)

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B7-1 Protein, Mouse, Recombinant (Fc Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized human CTLA4 (cat: 11159-H08H) at 10 μg/mL (100 μl/well) can bind mouse CD80-Fc (cat: 50446-M02H), The EC50 of mouse CD80-Fc (cat: 50446-M02H) is 28 ng/mL.
タンパク質の構築
A DNA sequence encoding the mouse CD80 (Q00609-1) extracellular domain (Met 1-Lys 245) was fused with the Fc region of human IgG1 at the C-terminus.
NCBI Reference No.
発現ホスト
HEK293 Cells
Mouse
予測N末端
Val 38
分子量
The recombinant mouse CD80 consists of 449 amino acids and has a predicted molecular mass of 50.7 kDa.
バッファー
Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

B7-1 Protein, Mouse, Recombinant (Fc Tag): 画像

B7-1 Protein, Mouse, Recombinant (Fc Tag): 別名

B71 Protein, Mouse; Cd28l Protein, Mouse; Ly-53 Protein, Mouse; Ly53 Protein, Mouse; MIC17 Protein, Mouse; TSA1 Protein, Mouse

B7-1 背景情報

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD8, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD8 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
完全な名称
CD80 molecule
参考文献
  • Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.
  • Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.
  • Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
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