Anti-Nectin-2 Antibody

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Anti-Nectin-2 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-Nectin-2 Antibody
検証済のアプリケーション
WB,ELISA,IP
交差反応
Reacts with: Human
特異性
Human Nectin-2
免疫原
Recombinant Human CD112 / Nectin2 protein (Catalog#10005-H08H)
調製
Produced in rabbits immunized with purified, recombinant Human CD112 / Nectin-2 extracellular domain (rhCD112; Catalog#10005-H08H; Met 1-Leu 360; NP_002847.1). CD112 specific IgG was purified by human CD112 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Nectin-2 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Nectin-2 Antibody (Rabbit Polyclonal 抗体) の画像

CD112 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

2 µL anti-CD112 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD112 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 58 kDa

Observed band size: 70 kDa

Anti-CD112 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Membrane Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:58 kDa

Observed band size:70 kDa

Anti-Nectin-2 Antibody: 別名

Anti-CD112 Antibody; Anti-HVEB Antibody; Anti-Nectin-2 Antibody; Anti-PRR2 Antibody; Anti-PVRR2 Antibody

Nectin-2 背景情報

Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
完全な名称
poliovirus receptor-related 2 (herpesvirus entry mediator B)
参考文献
  • Bachelet I, et al. (2006) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J Biol Chem. 281(37): 27190-6.
  • Wang L, et al. (2009) Molecular cloning, characterization and three-dimensional modeling of porcine nectin-2/CD112. Vet Immunol Immunopathol. 132(2-4): 257-63.
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