Anti-BCAM Antibody

Cat: 80222-RP02

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Anti-BCAM Antibody (Rabbit Polyclonal antibody) General Information

Product name
Anti-BCAM Antibody
Validated applications
ELISA,IHC-P
Species reactivity
Reacts with: Rat
Specificity
Rat BCAM
Immunogen
Recombinant Rat BCAM protein (Catalog#80222-R08H)
Preparation
Produced in rabbits immunized with purified, recombinant Rat BCAM (rR BCAM; Catalog#80222-R08H; Q9ESS6; Met1-Ala543). BCAM specific IgG was purified by Rat BCAM affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Anti-BCAM Antibody (Rabbit Polyclonal antibody) Validated Applications

Application Dilution Notes
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-BCAM Antibody (Rabbit Polyclonal antibody) Images

Rat BCAM / CD239 Immunohistochemistry(IHC) 3142
Immunochemical staining of rat BCAM in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).

BCAM Background Information

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.

Full Name
basal cell adhesion molecule (Lutheran blood group)
References
  • Kikkawa Y, et al. (2005) Review: Lutheran/B-CAM: a laminin receptor on red blood cells and in various tissues. Connect Tissue Res. 46 (4-5): 193-9.
  • El Nemer W, et al. (2007) Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood. 109 (8): 3544-51.
  • Colin Y, et al. (2008) Red cell and endothelial Lu/BCAM beyond sickle cell disease. Transfus Clin Biol. 15 (6): 402-5.
  • El Nemer W, et al. (2008) Role of Lu/BCAM in abnormal adhesion of sickle red blood cells to vascular endothelium. Transfus Clin Biol. 15 (1-2): 29-33.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-BCAM Antibody Rabbit Monoclonal IHC-P 80222-R005
    Anti-BCAM Antibody Rabbit Monoclonal ELISA 80222-R113
    Anti-BCAM Antibody Rabbit Polyclonal ELISA 80222-RP01
    Anti-BCAM Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 10238-RP02
    Anti-BCAM Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10238-R002
    Anti-BCAM Antibody Rabbit Polyclonal ELISA 10238-RP01
    Anti-BCAM Antibody Mouse Monoclonal ELISA,ELISA(Cap) 10238-MM01T
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