Anti-Myc tag Antibody

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Anti-Myc tag Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-Myc tag Antibody
検証済のアプリケーション
WB,IP
特異性
Myc tag
免疫原
A synthetic Myc tag peptide conjugated to KLH
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic Myc tag peptide conjugated to KLH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #08
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Myc tag Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1/5000-1/10000
IP IP:1-5μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Myc tag Antibody (Mouse Monoclonal 抗体) の画像

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: GST-myc(Recombinant protein) (5ng)

Lane B: GST-myc(Recombinant protein) (1ng)

Lane C: myc-GST(Recombinant protein) (100ng)

Lane D: myc-GST(Recombinant protein) (20ng)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

Anti-Myc Tag mouse monoclonal antibody at 1:5000 dilution.

Lane A: HA-mFABP4-myc transfected 293 cell lysate (10ug)

Lane B: myc-ARG1-HA transfected 293 cell lysate (5ug)

Lane C: HA-ARG1-myc transfected 293 cell lysate (10ug)

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution

Developed using the Odyssey technique.

Performed under reducing conditions.

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