Anti-DYKDDDDK (FLAG® epitope Tag) Antibody

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Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody
検証済のアプリケーション
ICC/IF,IF
特異性
DYKDDDDK (FLAG® epitope Tag)
免疫原
A synthetic peptide corresponding to the flag-tag sequence (DYKDDDDK).
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG2b Clone #01
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:50-1:1000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-DYKDDDDK (FLAG® epitope Tag) Antibody (Mouse Monoclonal 抗体) の画像

Immunofluorescence staining of flag-Tag in 293 cells, transfected with PCMV-CDH1-flag (Figure A), pSTEP2-Flag-FABP4-GST (Figure B), pSTEP2-Flag-ARG1-GST (Figure C), Flag-PRMT5-His (Figure D),Flag-PRMT6-His (Figure E) and His-flag-CD38(Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-flag-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
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