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人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone in cloning vector

    データシートレビュー関連製品プロトコル
    人々 YWHAS/SFN cDNA クローン製品情報
    Gene_bank_ref_id:NM_006142.3
    cDNA サイズ:747bp
    cDNA の説明:Full length Clone DNA of Homo sapiens stratifin.
    遺伝子の別名:YWHAS, SFN
    :Human
    ベクター:pMD18-T Simple Vector
    Plasmid:pMD-SFN
    制限サイト:
    タグ シーケンス:
    シーケンスの説明:Identical with the Gene Bank Ref. ID sequence except for the point mutation 746 G/A not causing the amino acid variation.
    Sequencing primers:M13-47 and RV-M
    ( We provide with SFN qPCR primers for gene expression analysis, HP100737 )
    Promoter:
    Application:
    Antibiotic in E.coli:Ampicillin
    Antibiotic in mammalian cell:
    Shipping_carrier:Each tube contains lyophilized plasmid.
    保存:The lyophilized plasmid can be stored at room temperature for three months.
    pMD18-T Simple Vector Information

    pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

    pMD18-T Simple Usage Suggestion

    The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

    Vector Sequence Download
    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone in cloning vector on other vectors
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    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-Flag タグHG10838-NFJPY47150
    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-His タグHG10838-NHJPY47150
    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-Myc タグHG10838-NMJPY47150
    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmid, N-HA タグHG10838-NYJPY47150
    人々 14-3-3 sigma/Stratifin/YWHAS Gene ORF cDNA clone expression plasmidHG10838-UTJPY47150
     発現ベクターの詳細情報
    Product nameProduct name
    背景

    14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.

    Size / Price
    カタログ: HG10838-M
    定価: 
    価格:      (You Save: )

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