|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-SFN rabbit polyclonal antibody at 1:500 dilution
Lane A: HeLa Whole Cell Lysate
Lane B: A549 Whole Cell Lysate
Lane C: PC12 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:27 kDa
Observed band size:33 kDa
(We are unsure as to the identity of these extra bands.)
SFN was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate
Lane B:0.5 mg A549 Whole Cell Lysate2 µL anti-SFN rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-SFN rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 27 kDa
Observed band size: 32 kDa
14-3-3 protein sigma (YWHAS), also known as stratifin (SFN) and epithelial cell marker protein 1, is a member of the14-3-3 proteins which are a family of conserved regulatory molecules expressed in all eukaryotic cells. The name 14-3-3 refers to the particular elution and migration pattern of these proteins on DEAE-cellulose chromatography and starch-gel electrophoresis. The 14-3-3 proteins eluted in the 14th fraction of bovine brain homogenate and were found on positions 3.3 of subsequent electrophoresis. There are seven genes that encode 14-3-3s in most mammals. 14-3-3 proteins have been identified as adapter proteins implicated in the regulation of a large spectrum of both general and specialized signaling pathway. More than 100 signaling proteins have been reported as 14-3-3 ligands including kinases, phosphatases, and transmembrane receptors, and the binding generally results in the modulation of the activity of the binding partner. YWHAE exists as a homodimer and present mainly in tissues enriched in stratified squamous keratinising epithelium. YWHAS has been repoted to interact with KRT17 and GAB2, and may regulate protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway upon binding to KRT17. Additionally, YWHAS (SFN) may also act as a p53-regulated inhibitor of G2/M progression.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-14-3-3 sigma Antibody||Rabbit||Polyclonal||ELISA||50576-RP01|
|Anti-14-3-3 sigma Antibody||Mouse||Monoclonal||FCM||10838-MM02|
|Anti-14-3-3 sigma Antibody (PE)||Mouse||Monoclonal||FCM||10838-MM02-P|
|Anti-14-3-3 sigma Antibody||Rabbit||Polyclonal||ELISA||10838-RP01|
|Anti-14-3-3 sigma Antibody||Rabbit||Polyclonal||ELISA||10838-RP02|