14-3-3 eta Protein, Human, Recombinant (GST Tag)

Cat: 10847-H09E

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14-3-3 eta Protein, Human, Recombinant (GST Tag) Product Information

Purity
> 90 % as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Activity
Testing in progress
Protein Construction
A DNA sequence encoding the human YWHAH (Q04917) (Gly 2-Asn 246) was fused with the GST tag at the N-terminus.
Accession#
Expressed Host
E. coli
Species
Human
Predicted N Terminal
Met
Molecule Mass
The recombinant human YWHAH/GST chimera consists of 476 amino acids and has a predicted molecular mass of 55 kDa as estimated in SDS-PAGE under reducing conditions.
Formulation
Lyophilized from sterile 20mM Tris, 0.15M NaCl, 20mM GST, pH 8.0
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Stability & Storage
Samples are stable for up to twelve months from date of receipt at -70℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

14-3-3 eta Protein, Human, Recombinant (GST Tag) in SDS-PAGE

Human YWHAH Protein 9164

14-3-3 eta Protein, Human, Recombinant (GST Tag) Alternative Names

YWHA1 Protein, Human

14-3-3 eta Background Information

Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
References
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
  • Other 14-3-3 eta Proteins

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