Anti-14-3-3 eta Antibody

Cat: 10847-RP02

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Anti-14-3-3 eta Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-14-3-3 eta Antibody
Validated applications
Species reactivity
Reacts with: Human
Human 14-3-3 eta
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
Produced in rabbits immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). 14-3-3 eta / YWHAH specific IgG was purified by Human 14-3-3 eta / YWHAH affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 eta Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 eta Antibody (Rabbit Polyclonal antibody) Images
Human YWHAH Western blot (WB) 13294

Anti-YWHAH rabbit polyclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:31 kDa

Human YWHAH Immunoprecipitation(IP) 17678

YWHAH was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

Lane C:0.5 mg k562 Whole Cell Lysate

Lane D:0.5 mg NIH-3T3 Whole Cell Lysate

2 µL anti-YWHAH rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH rabbit polyclonal antibody,at 1:2000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta Antibody Alternative Names
Anti-YWHA1 Antibody
14-3-3 eta Background Information
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 eta Antibody Mouse Monoclonal WB,ELISA,IP 10847-MM06
    Anti-14-3-3 eta Antibody Rabbit Polyclonal ELISA 10847-RP01
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