Anti-14-3-3 eta Antibody

Cat: 10847-MM06

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Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-14-3-3 eta Antibody
Validated applications
Species reactivity
Reacts with: Human
Human 14-3-3 eta
No cross-reactivity in ELISA with E.coli cell lysate
Recombinant Human 14-3-3 eta / YWHAH protein (Catalog#10847-H09E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 eta / YWHAH (rh 14-3-3 eta / YWHAH; Catalog#10847-H09E; Q04917; Gly2-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #06
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 eta Antibody (Mouse Monoclonal antibody) Images
Human YWHAH Western blot (WB) 13293

Anti-YWHAH mouse monoclonal antibody at 1:500 dilution

Lane A: U937 Whole Cell Lysate

Lane B: 293T Whole Cell Lysate

Lane C: Jurkat Whole Cell Lysate

Lane D: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:28 kDa

Observed band size:30 kDa

Human YWHAH Immunoprecipitation(IP) 17677

YWHAH was immunoprecipitated using:

Lane A:0.5 mg Jurkat Whole Cell Lysate

Lane B:0.5 mg k562 Whole Cell Lysate

2 µL anti-YWHAH mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-YWHAH mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Anti-14-3-3 eta Antibody Alternative Names
Anti-YWHA1 Antibody
14-3-3 eta Background Information
Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta
  • Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6.
  • Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20.
  • Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40.
  • Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 eta Antibody Rabbit Polyclonal WB,ELISA,IP 10847-RP02
    Anti-14-3-3 eta Antibody Rabbit Polyclonal ELISA 10847-RP01
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