All 14-3-3 epsilon reagents are produced in house and quality controlled, including 2 14-3-3 epsilon Antibody, 45 14-3-3 epsilon Gene, 1 14-3-3 epsilon IPKit, 3 14-3-3 epsilon Protein, 3 14-3-3 epsilon qPCR. All 14-3-3 epsilon reagents are ready to use.
Recombinant 14-3-3 epsilon proteins are expressed by E. coli with fusion tags as N-cleavage, N-GST.
14-3-3 epsilonantibodies are validated with different applications, which are ELISA, WB, ICC/IF, IF, IP.
14-3-3 epsiloncDNA clones are full length sequence confirmed and expression validated. There are 13 kinds of tags for each 14-3-3 epsilon of different species, especially GFP tag, OFP tag, FLAG tag and so on. There are three kinds of vectors for choice, cloning vector, expression vector and lentivrial expression vector.
YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.