Anti-14-3-3 beta Antibody

Cat: 10843-MM03
Contact us:
All 14-3-3 beta Reagents
CRO Services
Product recommended

All 14-3-3 beta Reagents
Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-14-3-3 beta Antibody
Validated applications
Species reactivity
Reacts with: Human
Human 14-3-3 beta
Has cross-reactivity in ELISA with Cynomolgus YWHAB
No cross activity in ELISA with E.coli cell lysate (BL21 cell line)
Recombinant Human 14-3-3 beta / YWHAB protein (Catalog#10843-H09E)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human 14-3-3 beta / YWHAB (rh 14-3-3 beta / YWHAB; Catalog#10843-H09E; NP_003395.1; Met1-Asn246). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #03
Protein A
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-14-3-3 beta Antibody (Mouse Monoclonal antibody) Images
Human 14-3-3 beta/YWHAB Immunofluorescence(IF) 2835
Immunofluorescence staining of Human YWHAB in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-Human YWHAB monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human 14-3-3 beta/YWHAB Flow Cytometry (FC) 4988
Flow cytometric analysis of Human YWHAB expression on Molt-4 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human YWHAB, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Anti-14-3-3 beta Antibody Alternative Names
Anti-GW128 Antibody;Anti-HEL-S-1 Antibody;Anti-HS1 Antibody;Anti-KCIP-1 Antibody;Anti-YWHAA Antibody
14-3-3 beta Background Information

14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.

Full Name
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
  • Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
  • Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
  • Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-14-3-3 beta Antibody Mouse Monoclonal WB,ELISA,IP 90022-MM05
    Anti-14-3-3 beta Antibody Rabbit Polyclonal WB,IP 90022-T36
    Anti-14-3-3 beta Antibody Rabbit Polyclonal ELISA 10843-RP01
    Anti-14-3-3 beta Antibody Rabbit Polyclonal WB,ELISA,IP 10843-RP02
    Anti-14-3-3 beta Antibody (APC) Mouse Monoclonal FCM 10843-MM03-A
    Anti-14-3-3 beta Antibody (FITC) Mouse Monoclonal FCM 10843-MM03-F
    Anti-14-3-3 beta Antibody (PE) Mouse Monoclonal FCM 10843-MM03-P
    Anti-14-3-3 beta Antibody (PerCP) Mouse Monoclonal FCM 10843-MM03-C
    info info
    添加购物车成功! 添加购物车失败!